The first step in screening a phage library is to titre the stock to find the concentration of phage particles present. This was performed in your first lab – the phage plates you will be provided ...
The MSA will select for organisms such as Staphylococcus species which can live in areas of high salt concentration (plate on the left in the picture below). This is in contrast to Streptococcus ...
Having spent considerable time on preparation of ‘bottom’ agar plates for the agar layer assay by the plaque count method 1,2 and for high-titre bacterio-phage stock preparation on a large ...
Spirit blue agar contains an emulsion of olive oil and spirit blue dye. Bacteria that produce lipase will hydrolyze the olive oil and produce a halo around the bacterial growth. The Gram-positive rod, ...