This protocol describes the transfer of RNA from agarose gels to nylon ... of Plexiglas or a glass plate to form a support that is longer and wider than the gel. Drape the ends of the blotting ...

1. Prepare a 40 ml 1 % agarose gel in 0.5X TBE, containing 0.5 µg/ml ethidium bromide. Each gel will be shared by 2 groups on the same bench. 3. Each group will load PCR and control samples on one ...

Analyze the size and quality of the products by electrophoresis through an agarose gel. Select several colonies ... Reactions are performed in a 96-well plate and are cleaned up for sequencing ...