尤其在癌症研究领域,CRISPR-Cas9开启了基因筛选(Genetic Screening)的新时代。通过引入单向导RNA(sgRNA),CRISPR能够高通量地在细胞中敲除特定基因 ...
大家都知道,CRISPR/Cas9基因编辑技术在高效获得基因敲除突变(knockout)方面是非常有效的,但是想要通过同源重组修复途径 ...
CRISPR-Cas9技术因其高效的基因编辑能力 ... 这一技术的核心在于通过随机激活sgRNA和在同一单细胞克隆内生成内部对照(Internal Control),在保持实验 ...
1月9日,记者从陆军军医大学西南医院获悉,该院检验科团队提出,可通过纳米技术,让控制成簇的规律间隔短回文重复序列/相关蛋白9(CRISPR/Cas9)实现精准释放,精准识别抗癌基因,提升治疗效果,为肝癌精准治疗提供全新手段。相关研究成果于近日刊发于国际学术期刊《美国化学学会纳米杂志》(ACSNano)。
博生物自主开发的riboEDIT™ CRISPR-Cas9体系是采用天然复合物系统(crRNA和tracrRNA)进行基因编辑。 锐博提供基于Cas9 mRNA的全RNA体系和基于Cas9蛋白的RNP体系,tracrRNA可实现大规模合成,靶向DNA目的序列的crRNA可以高通量合成,针对哺乳动物细胞可简单通过化学转染或 ...
image: 报告RNA富集的双sgRNA核蛋白(reporter RNA enriched dual-sgRNA/CRISPR-Cas9 ribonucleoproteins, RE-DSRNP)高效编辑技术体系由双sgRNA编辑系统和报告RNA富集的核糖 ...
When the CRISPR Cas9 protein is added to a cell along with a piece of guide RNA, the Cas9 protein hooks up with the guide RNA and then moves along the strands of DNA until it finds and binds to a ...
One of bacterial cells' most well-known defenses against these viruses is the CRISPR system ... a guide RNA that directs an enzyme called Cas9 to snip the viral DNA, preventing infection.
Recent research published in the journal Science Bulletin detailed the creation of a tool called ChCas12b that can accurately edit genomes and identify single-base mutations. The research team of ...
CRISPR Therapeutics AG is a gene editing company, which engages in the development of transformative gene-based medicines for serious diseases using its proprietary CRISPR/Cas9 platform.