The core components of CRISPR-based genome-editing therapies are bacterial proteins called nucleases that can stimulate ...

Type V and VI CRISPR/Cas systems have emerged as promising cutting-edge diagnostic tools for nucleic acid detection. Nevertheless, the challenge of low target concentrations necessitates ...

Professor Kevin J. Zwezdaryk and Chandler H. Monk discuss CRISPR and diagnostics, focusing on the development of a portable ...

Recent research published in the journal Science Bulletin detailed the creation of a tool called ChCas12b that can accurately edit genomes and identify single-base mutations. The research team of ...

The Cas12 enzymes are distinctly different from their Cas9 counterparts ... Huang, Z. & Patel, D. J. (2016). Type V CRISPR-Cas Cpf1 endonuclease employs a unique mechanism for crRNA-mediated target ...

麻省理工学院Broad研究所、哈佛大学和赛勒斯生物技术公司的研究人员现在已经设计了两种CRISPR核酸酶，Cas9和Cas12，以掩盖它们对免疫系统的影响。该团队确定了每个核酸酶上触发免疫系统的蛋白质序列，并使用计算建模来设计逃避免疫识别的新版本。与小鼠的标准核酸酶相比，工程酶具有相似的基因编辑效率和降低的免疫反应。