A Sanger sequencing reaction is run with a single primer. The following table lists the recommended amount of DNA template and primer for optimal Sanger sequencing results. Note: for plasmid DNA the ...

thus allowing polymerases access to the DNA template. However, the presence of nucleosomes and the folding of chromatin into 30-nanometer fibers pose barriers to the enzymes that unwind and copy DNA.

Because pol-a/primase always requires CMG function to create the ssDNA template, it could never surpass the DNA helicase (Figure 2b). Without a connecting link, the CMG complex could just "run off ...

Determining the number of copies of a double stranded DNA template (be it genomic DNA, plasmid or an amplified fragment) is essential for many genetic quantification applications in research and ...

These can be arranged into a desired order and then used to 'print' their data onto DNA template strands. Rather than writing one bit at a time, this printing press speeds it up to 350 bits ...

In the era of big data, global mass data flow has presented data storage systems with a looming challenge. As DNA has incredibly high storage density – a single gram of DNA can store 215,000 terabytes ...

However, the idea that replication forks would form at origins of DNA replication and proceed without impairment to copy the chromosomes has proven naive. It is now clear that replication forks stall ...

Briola and co-authors determined the structure of the human CTF18 clamp loader bound to PCNA to high resolution, analyzed the structure, and tested a new mechanism involving a human-specific Ctf18 ...

During DNA replication, the double helix unwinds, and each strand serves as a template for synthesizing a new complementary strand. Adenine on the template strand will always pair with thymine on the ...