Optically pure alcohols are prevalent motifs in natural products and pharmaceuticals, driving the development of effective ...

The fleeting union of the enzyme and the substance on which it acts holds a key to our understanding of life processes. Many ingenious techniques are in use today to isolate it for study ...

We had at our disposal not only an enzyme assay but also a highly discriminatory ... the other carboxyl was bound to an arginine moiety like the substrate carboxyl group. Therefore, in our ...

Protein acylation is a critical area of research within the broader field of post-translational modifications, involving the addition of acyl groups to ...

Each enzyme molecule has a special place called the active site where another molecule, called the substrate, fits. The substrate goes through a chemical reaction and changes into a new molecule ...

However, it is safe to say that no enzyme is perfect in the sense of catalyzing only a single reaction with a single substrate. For enzymes such as P450s and glutathione S-transferases that are ...

This causes the formation of more enzyme-substrate complexes, leading to an increase in enzyme activity. This means the active site loses its important shape and can no longer form enzyme ...

The process of an enzyme breaking a substrate molecule apart. If enzymes are heated too much or put into a higher or lower pH, their shape can change. The enzyme undertakes a process of ...

activity assays determined that GnT-IVa enzyme with GlcNAc glycan branches (higher binding) near the lectin domain decreases ...

Use a pair of plastic cuvettes, and start with the most dilute sample. 4. Prepare the substrate solution (3 tablets = 15 mg p-nitrophenylphosphate in 10 ml citrate buffer, = 5.5 mM). 5. Now you have ...