We subcloned the cDNA encoding Factor IX into the same vector used to express luciferase protein. We transiently transfected CHO cells with 3:6 (plasmid DNA/L-PEI) polyplex ratio. We obtained ...

A newly developed luciferase-based reporter can detect problems in protein translocation and disulfide bond formation in the endoplasmic reticulum (ER), as reported by researchers at Science Tokyo.

Bioluminescence is widely used for in vivo imaging of nude mice. By conjugating luciferase protein to quantum dots, bioluminescence resonance energy transfer (BRET) turns these useful fluorophores ...

These cell lines were established through single cell cloning, and the cells constitutively express high levels of enzymatically active luciferase protein, which can be detected via in vitro and ...

Development of luciferase-based highly sensitive reporters that detect ER-associated protein biogenesis abnormalities. iScience , 2024; 27 (11): 111189 DOI: 10.1016/j.isci.2024.111189 Cite This Page : ...

A team led by researchers at the University of Toronto has created a platform, called SIMPL2, that revolutionizes the study ...