A newly developed luciferase-based reporter can detect problems in protein translocation and disulfide bond formation in the endoplasmic reticulum (ER), as reported by researchers at Science Tokyo.

We subcloned the cDNA encoding Factor IX into the same vector used to express luciferase protein. We transiently transfected CHO cells with 3:6 (plasmid DNA/L-PEI) polyplex ratio. We obtained ...

Bioluminescence is widely used for in vivo imaging of nude mice. By conjugating luciferase protein to quantum dots, bioluminescence resonance energy transfer (BRET) turns these useful fluorophores ...

These cell lines were established through single cell cloning, and the cells constitutively express high levels of enzymatically active luciferase protein, which can be detected via in vitro and ...

SIMPL2 is an update of SIMPL that involves the use of the split luciferase enzyme for detection of protein interactions through luminescence. In addition to improving identification of ...

SIMPL2 is an update of SIMPL that involves the use of the split luciferase enzyme for detection of protein interactions through luminescence. In addition to improving identification of ...

Protein synthesis in eukaryotic cells found ... natural processes and created a reporter molecule based on firefly luciferase (FLuc) that functions similarly. When oxygen, adenosine triphosphate ...

Computational pipeline SPICE systematically screens and predicts novel protein-protein binding complexes including the previously unrecognized global association and functional cooperation between ...

SIMPL2 is an update of SIMPL that involves the use of the split luciferase enzyme for detection of protein interactions through luminescence. In addition to improving identification of ...