A new study by Tel Aviv University reveals how bacterial defense mechanisms can be neutralized, enabling the efficient ...

In step 2, the gene fragment is integrated into a circular piece of DNA, called a plasmid, which is the main delivery vehicle into the cell and contains an antibiotic resistance gene for plasmid ...

To prepare the cloning vectors, transform separately pET11a-link-NGFP and pMRBAD-link-CGFP into an E. coli strain that gives high-quality plasmid DNA (for example, DH10B or XL1-Blue) and plate ...

CHICAGO--(BUSINESS WIRE)--Genetic Immunity, a leader in plasmid DNA-based immunotherapies ... freeing them from the tedious work of cloning and packaging vectors in the lab.

The first breakthrough is that it eliminates the requirement to clone DNA fragments into bacteria. Consequently, the Genome Sequencer FLX avoids the cloning bias that is introduced in sample ...

Included are restriction mapping, cloning, plasmid purification, blot hybridization, and DNA sequencing. Students are introduced to computer software utilized for DNA sequence analysis and ...