Although DNA is often found as a single-stranded polynucleotide ... This means that one strand's sugar-phosphate chain runs in the 5' to 3' direction, whereas the other's runs in the 3' to ...

As the helicase moves along the DNA molecule, it continues breaking these hydrogen bonds and separating the two polynucleotide chains (Figure 1). Meanwhile, as the helicase separates the strands ...