All steps of ISH – from pre-hybridization to additions of probes, hybridization, stringency washes and antibody-mediated chromogenic or fluorescent reaction – are carried out by the robotic system.
The GeneChip® Hybridization Oven 645 provides precise temperature and rotation control to ensure the successful hybridization of as many as 64 GeneChip®-brand cartridges at one time. An ...
(a) The basic elements of FISH are a DNA probe and a target sequence. (b) Before hybridization, the DNA probe is labeled by various means, such as nick translation, random primed labeling ...
In addition, the probe has a label, which is typically a radioactive atom or a fluorescent dye. Thus, following hybridization, the probe permits the RNA molecule of interest to be detected from ...
This core performs non-radioactive, RNA in situ hybridization (ISH) on tissue sections ... preparation of frozen and paraffin sections, preparation of RNA probes from customer templates, conducting ...