Immunodetection techniques are based ... needs to specifically recognize your protein. If you’re using immunofluorescence or immunohistochemistry to stain cells or tissues, it may be a good ...

1| Incubate the cell lysate with 50 μl of a 50% slurry of glutathione-agarose beads and 25 μg of GST for 2 h at 4 °C with end-over-end mixing. The amount of lysate needed to detect an ...