Solubilization buffer (SB): 200 mM Tris-HCl (pH 8.8), 20% Glycerol, 5 mM EDTA (pH 8.0), 0.02% bromphenol blue, make aliquots of 700 μl and keep at −20 °C. Before use, add 200 μl 20% SDS and ...

and unassembled GFP-fragment fusions are typically insoluble, the reassembled fusion can be purified and concentrated in a single step from the soluble fraction of cell lysate 7. Combine the ...