Solubilization buffer (SB): 200 mM Tris-HCl (pH 8.8), 20% Glycerol, 5 mM EDTA (pH 8.0), 0.02% bromphenol blue, make aliquots of 700 μl and keep at −20 °C. Before use, add 200 μl 20% SDS and ...
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