The situation is even more complicated because the splicing code can produce multiple outcomes in a given cell type and can be interpreted differently in different cellular environments.

How can only 25,000-30,000 protein-coding genes in humans produce the massive variety of proteins, cells, and tissues that exist in our bodies? The answer: alternative splicing.

However, this raised an intriguing question: does disease-relevant genetic regulation of splicing occur only in one or a few cell types? To answer this research question, the main bottleneck is ...