Blood mononuclear cells (HNC) are commonly isolated by Ficoll-Hypaque density centrifugation (FH) prior to monoclonal antibody staining and flow cytometric ... by FH and a whole blood technique ...

In this presentation, we will propose a method to phenotype whole blood ILCs and PBMC ILCs. We will present how to use the new CytoFLEX flow cytometer to look into these different human classical ILC.