To determine which aliquot of template (1 μl, 5 μl or 10 μl) yields the best range of amplified products, analyze the reaction products by agarose gel electrophoresis, including as control 1 ...

The PCR products were analyzed on 2% agarose gel stained with ethidium bromide. In conclusion, cytogenetic abnormalities identified by karyotyping remain an important prognostic factor in AML. The ...

We centrifuged the suspension at 13,000 r.p.m. for 10 min, resuspended the pellet in 100 μl somatic cell lysis buffer (0.01 M Tris, pH 8.0, 0.01 M EDTA, 0.1 M NaCl, 2% SDS and 20 μg/ml ...

Amplicons were recovered from 2% agarose gels and purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, USA) according to manufacturer’s instructions and quantified using a ...

RNAs in plants and cnidarian animals operate similarly by binding their targets with nearly-full complementarity, in contrast ...

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The PCR-amplified fragment of each bisulfite-treated DNA sample was gel-purified using the Zymoclean Gel DNA Recovery Kit (Zymo Research) and cloned into the pGEM-T Easy vector (Promega, Madison, WI).

To ensure the differences in the observed DNA-stimulated ATPase were not due to differences in DNA-binding affinity (Fig. 1C), we examined the binding of tetrameric and pentameric complexes to DNA ...

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