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苏昕课题组开发特异性靶向双链核酸序列的新工具
双链核酸的序列特异性靶向是分子诊断和原位成像的重要组成部分。CRISPR相关蛋白(Cas)在crRNA的引导下,通过识别原间隔区相邻基序(PAM)来切割靶标核酸。然而,CRISPR-Cas系统对PAM的依赖性以及脱靶效应不仅限制了其靶标范围,且影响了 ...
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