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For the Western Blot, the monoclonal antibody was diluted at 1:1000 and the secondary antibody was diluted at 1:5000. The concentration of the mAbs was quantified using the BCA protein assay kit ...
Protein concentrations were then estimated by BCA assay (ThermoFisher Pierce, Rockford, IL). Disulfide bonds were reduced by incubation with 5 mM DTT (45 min, 37°C), followed by alkylation of cysteine ...
For examining the malondialdehyde, total cell contents were extracted and processed according to the instruction of lipid peroxidation malondialdehyde assay kit (S0131S, Beyotime, Beijing, China). The ...
Calculations were performed as per manufacturer’s instructions. The resultant data was normalized to total protein, which was assessed using Pierce™ BCA Protein Assay Kit (Thermo Scientific™ cat # ...
An improved assay, the Thermo Scientific™ Pierce™ BCA Protein Assay using bicinchoninic acid, is a chelator-based assay with exceptional linearity and low protein-to-protein variability that was ...
Western blot analysis was carried out using standard procedures. Protein levels of the purified γ-secretase enzyme and the C100 substrate were quantified using a BCA assay (Thermo Fisher Scientific, ...
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