Ltd. (Mumbai, India) and Malachite green (MG) and Bromophenol blue (BB) were purchased from Merck Specialities Private Limited (Mumbai, India) and Qualigens Fine Chemicals Ltd. (Mumbai, India) ...

Cell pellets were resuspended in Laemmli buffer [62.5 mM Tris-HCl (pH 6.8), 2% w/v SDS, 10% glycerol, 50 mM DTT, 0.01% w/v bromophenol blue], sonicated, denatured for 5 min and separated on SDS-PAGE.