CRISPR-Cas9技术因其高效的基因编辑能力成为 ... 在这一技术中，研究人员利用Cre重组酶（CreERT2）将每个sgRNA分为活跃（Active）和非活跃（Inactive）两种 ...

CRISPR-Cas9 genome editing exploits the CRISPR-Cas system to modify a genome in a targeted manner. Guided by RNA, the Cas9 endonuclease breaks DNA at a target sequence. Imprecise repair of the ...

When the CRISPR Cas9 protein is added to a cell along with a piece of guide RNA, the Cas9 protein hooks up with the guide RNA and then moves along the strands of DNA until it finds and binds to a ...

Schematic of the intron targeting-mediated strategy for generating hey2zCKOIS zebrafish by using the CRISPR/Cas9 system ... Ki(flk1-P2A-Cre) embryos at 3.5 dpf. Figure 1D. In the absent of ...