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清华大学揭示Cas12e蛋白的盐敏感性
在本研究中,Cas12e同源蛋白展现出了多样的单链DNA反式切割活性,其中PlmCas12e具有最高效的反式切割活性。当盐浓度降低时,其活性进一步增强。这些结果展现了Cas12e家族蛋白在核酸检测中的巨大潜力,并为优化其检测性能提供了理论基础。
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