So the question remains "What are the essential prerequisites for establishing a quantitative RT-PCR assay?" To date, a great deal of effort has been spent defining the "correct" conditions that ...

Users can search the database by gene, species or type of assay (TaqMan, SYBR Green I or RT-PCR) to find the primer's location, sequence and original reference. Similarly, the RTPrimerDB database ...

High CDC20 expression promotes aneuploid cancer cell sensitivity to spindle assembly checkpoint (SAC) inhibition by ...

RT-PCR analysis of serum samples from 792 participants, collected between May and July from 2022 to 2024 identified 26 ...

Future Virology. 2008;3(2):179-192. Although not listed as a hemorrhagic fever virus, illness caused by CHIKV can be confused with diseases such as dengue or yellow fever, based on the similarity ...

Positive 31.1 Not assessed Not assessed Specimens were tested with an influenza A virus–specific reverse-transcriptase–polymerase-chain-reaction (RT-PCR) assay. Specimens were analyzed for ...

So how can you be sure you have the flu? As with COVID, testing is the only way to confirm the presence of the influenza ...

Two false negatives were detected, one from a sample with a late Ct value of 35 in diagnostic RT-PCR, indicating that an active infection was no longer present. The PLpro assay is easily scalable and ...

PCR-Machines are based on Polymerase Chain Reaction (PCR) and produce copies of genes exponentially. The PCR cycling reactions are carried out in an automated thermocycler, which is capable of ...

Mean cycle threshold (Ct) values for RT-PCR assay results from positive specimens were also reported; specimens with Ct values of less than 38 with the use of the CDC influenza A/H5 subtyping ...

This application note explores how an innovative panel provides a comprehensive tool for infectious disease research and ...

Methods—Mucosal biopsy specimens and lamina propria cells from normal and IBD colons were studied by reverse transcription polymerase chain reaction (RT-PCR), western blot analysis, and ELISA (enzyme ...