Developed in the late 1980s, liposomal transfection reagents work by enveloping the DNA or RNA and then fusing with the cell membrane to deposit the nucleic acid cargo inside. Roche Applied ...

1| Harvest exponentially growing cells 24 h before transfection by trypsinization and replate them at a density of 1 × 10 5 to 4 × 10 5 cells/cm 2 in 60-mm tissue culture dishes or in 12-well ...