The human OS cell lines MG63 and U2OS were used for the functional assays. Cell proliferation and migration were analyzed by the EdU assay and Transwell assay respectively. CLK1 protein expression was ...
For basolateral delivery, transwell cultures were inverted, and the basolateral surface was covered with 50 μL of each AAV capsid (MOI = 10,000) of serum-free medium and vector. Cells were incubated ...
The expressions of CCL2 and CCR2 in decidua and peripheral blood were detected by immunohistochemistry, enzyme-linked immunosorbent assay and flow cytometry. Transwell was used to detect the ...