(St. Louis, MO, USA). The DMEM medium was purchased from HyClone Co. (Los Angeles, USA). The 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) was purchased from Solarbio Co.
Cell viability was tested using the methyl-thiazolyl-tetrazolium (MTT) reagent. Nitric oxide production in cell culture media was assessed by Griess reaction. Green Malachite Assay was used to ...
Transfection with miR-193b precursor and inhibitor were used to confirm targets of miR-193b. Proliferative effects of urokinase-type plasminogen activator (uPA) were determined by water-soluble ...
1 Department of Biological Sciences, Mycobacteria Research Laboratory, Institute of Structural and Molecular Biology, Birkbeck, University of London, London, UK 2 Department of Pharmaceutical and ...
All experimental protocols were approved by the Institutional Animal Care and Use Committee of the University of Alabama at Birmingham and performed in accordance with the National Institutes of ...
A convenient synthesis of the new enamine derivatives 2-(4-morpholinyl)-3-(3-methyl-2-butenyl)-1,4-naphthalenedione, 2-(1-piperidinyl)-3-(3-methyl-2-butenyl)-1,4 ...
A control group was prepared simultaneously and a growth curve was generated. Cell Viability Assay The effects of Noni extract treatment on cell viability were determined by MTT assay, which is based ...
After further 48 hr, the pyoverdine-containing culture medium was removed, and cells were incubated with 1 µg/mL thiazolyl blue tetrazolium bromide (Sigma, D5655) in 100 µL of fresh medium for 1.5 hr ...