(St. Louis, MO, USA). The DMEM medium was purchased from HyClone Co. (Los Angeles, USA). The 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) was purchased from Solarbio Co.
Cell viability was tested using the methyl-thiazolyl-tetrazolium (MTT) reagent. Nitric oxide production in cell culture media was assessed by Griess reaction. Green Malachite Assay was used to ...
Transfection with miR-193b precursor and inhibitor were used to confirm targets of miR-193b. Proliferative effects of urokinase-type plasminogen activator (uPA) were determined by water-soluble ...
1 Department of Biological Sciences, Mycobacteria Research Laboratory, Institute of Structural and Molecular Biology, Birkbeck, University of London, London, UK 2 Department of Pharmaceutical and ...
All experimental protocols were approved by the Institutional Animal Care and Use Committee of the University of Alabama at Birmingham and performed in accordance with the National Institutes of ...
NADH和四唑盐类化合物INT(2-p-iodophenyl-3-nitrophenyl tetrazolium chloride)在硫辛酰胺脱氢酶(Diaphorase)的催化下反应生成红色的甲臜(Formazan)。 (3)颜色变化与 ...
A convenient synthesis of the new enamine derivatives 2-(4-morpholinyl)-3-(3-methyl-2-butenyl)-1,4-naphthalenedione, 2-(1-piperidinyl)-3-(3-methyl-2-butenyl)-1,4 ...
在生物医学研究的广阔领域中,细胞活性检测扮演着至关重要的角色。它不仅帮助科学家们评估细胞的生存状态,还能深入 ...
A control group was prepared simultaneously and a growth curve was generated. Cell Viability Assay The effects of Noni extract treatment on cell viability were determined by MTT assay, which is based ...
After further 48 hr, the pyoverdine-containing culture medium was removed, and cells were incubated with 1 µg/mL thiazolyl blue tetrazolium bromide (Sigma, D5655) in 100 µL of fresh medium for 1.5 hr ...