An alternative to the immunolabelling methods is the cytochemical staining of horse radish peroxidase (HRP) tagged proteins or endocytosed HRP. HRP catalyzes an enzymatic reaction that yields an ...
Negative staining Gallery Image 1 Detection of Potyviral genome-linked protein VPg at only one end of the virions (Puustinen et al., 2002, J.Virol. 76:12703-12711) Image 2 Whole mount immunolabelling ...
Immunodetection techniques are based ... needs to specifically recognize your protein. If you’re using immunofluorescence or immunohistochemistry to stain cells or tissues, it may be a good ...
1| Incubate the cell lysate with 50 μl of a 50% slurry of glutathione-agarose beads and 25 μg of GST for 2 h at 4 °C with end-over-end mixing. The amount of lysate needed to detect an ...
The western blotting technique that began as a means of detecting the protein of interest in a ... labeling reagent that offers an attractive alternative to achieve total protein normalization. The ...
A team of researchers at the University of California, Los Angeles (UCLA) has introduced a virtual Gram staining technique that could transform how microbiologists stain and classify bacteria.