BCA Assay and Lowry Assays | Thermo Fisher Scientific - US
Explore our BCA assay kits, that provide high-precision, detergent compatible protein quantification determination.
BCA Principle: - Assay-Protocol
The Pierce BCA Protein Assay is a detergent-compatible formulation based on bicinchoninic acid (BCA) for the colorimetric detection and quantitation of total protein.
Bicinchoninic Acid Assay - an overview | ScienceDirect Topics
The Bicinchoninic Acid Assay is a protein quantification technique that utilizes a weak acid composed of two carboxylated quinoline rings to produce a purple end product that can be measured at 562 nm. It allows for the quantification of protein concentrations in a solution by comparing absorption spectra with those of known protein concentrations.
Protein Quantification: BCA Assay – QB3 Berkeley
The bicinchoninic acid (BCA) assay is one of many ways to measure the total protein concentration of an unknown sample. In this assay, copper (II) [Cu 2+ ] is reduced to copper (I) [Cu 1+ ] in the presence of peptides in alkaline solutions.
Protocol for Bicinchoninic Acid (BCA) Protein Assay
The Bicinchoninic Acid (BCA) protein assay is a reliable and sensitive method for the quantification of total protein in a sample. It is more sensitive than the Bradford assay and can detect protein concentrations as low as 0.5 μg/mL.
The BCA Protein Assay combines the protein-induced biuret reaction with the highly sensitive and selective colorimetric detection of the resulting cuprous cation (Cu1+) by bicinchoninic acid (BCA). A purple colored reaction product is formed by the chelation of two molecules of BCA with one cuprous ion. The
Protein Determination by the Bicinchoninic Acid (BCA) Method
A standard procedure for the quantitative determination of total protein concentration of a solution by the BCA method.
Total Protein Determination with Bicinchoninic Acid (BCA method)
The Pierce BCA Protein Assay is a detergent-compatible formulation based on bicinchoninic acid (BCA) for the colorimetric detection and quantitation of total protein.
The Bicinchoninic Acid (BCA) Assay for Protein Quantitation
Both a standard assay (0.1–1.0 mg protein/mL) and a microassay (0.5–10 µg protein/mL) are described. 1. Reagent A: sodium bicinchoninate (0.1 g), Na 2 CO 3 · H 2 O (2.0 g), sodium tartrate (dihydrate) (0.16 g), NaOH (0.4 g), NaHCO 3 (0.95 g), made up to 100 mL. If necessary, adjust the pH to 11.25 with NaHCO 3 or NaOH (see Note 1). 2.
Protein determination by the bca method - Rice University
Both a standard assay for concentrated proteins and a micro assay for dilute protein solutions are described below. BCA serves the purpose of the Folin reagent in the Lowry assay, namely to react with complexes between copper ions and peptide bonds to produce a purple end product.
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