Enhancing Antigen Retrieval To Unmask Signaling …
In this study, we tested the hypothesis that optimizing antigen retrieval methods will improve phosphoproteins unmasking and enhance their immunohistochemical staining signal. We screened 4 antigen retrieval methods by using antibodies specific for 3 oncogenic phosphoproteins to stain human lymphoma tumors that were developed in SCID mice and ...
Unmasking of phosphorylation-sensitive epitopes on p53 and
2000年7月5日 · Phosphorylation of one or more residues within an epitope recognized by a particular antibody may compromise the ability of that antibody to bind the target protein. Inhibition of...
Why Perform Epitope/Antigen Unmasking? - LabCE
FFPE tissues form hydroxymethyl groups that link to protein groups, which result in strong bonds that form between proteins and calcium ions called “methylene bridges.” These bridges create an epitope masking affect which needs to be removed or destroyed. This condition is easily remedied using epitope/antigen retrieval techniques.
Tips for Step 4: Perform Antigen/Epitope Retrieval - Bio-Rad
The effects of tissue fixation can be partially reversed by performing epitope retrieval, also known as antigen unmasking. Two types of epitope retrieval have been established; heat based (HIER; Heat-Induced Epitope Retrieval) or enzyme based (PIER; Proteolytic-Induced Epitope Retrieval). Table 1: Comparison of epitope retrieval methods.
Ex-chitin-g news on drug-induced fungal epitope unmasking
2023年9月22日 · Wagner et al. showed that calcineurin and the Mkc1p kinase regulate chitin deposition and β (1,3)-glucan unmasking. They further identified chitin synthesis as a key driving force in cell wall structure disruption leading to epitope exposure.
Unmasking Epitopes Using Antigen Retrieval for Formalin
2021年9月29日 · Protein in formalin-fixed tissue can undergo epitope masking, leading to reduction or elimination of antibody binding. Learn how antigen retrieval can reverse this masking.
Latent NOTCH3 epitopes unmasked in CADASIL and
2014年10月2日 · We describe the discovery of novel N-terminal NOTCH3 epitopes in diseased arteries in human CADASIL brains. We show that these epitopes are undetectable in native NOTCH3 protein, but can be unmasked specifically by disulfide bond disruption. Our studies suggest an important role of reduced cysteine in CADASIL pathogenesis. 2. Results. 2.1.
Ex-chitin-g news on drug-induced fungal epitope unmasking
2023年10月3日 · Wagner et al. showed that calcineurin and the Mkc1p kinase regulate chitin deposition and β (1,3)-glucan unmasking. They further identified chitin synthesis as a key driving force in cell wall...
[PDF] Unmasking the mysteries of antigen or epitope retrieval …
2004年2月1日 · Unmasking the mysteries of antigen or epitope retrieval and formalin fixation. It has revolutionized the practice of diagnostic immunohistochemical analysis, permitting the use of a much wider range of monoclonal and polyclonal antibodies on deparaffinized, formalin-fixed…
IHC Antigen Retrieval | Proteintech Group - ptglab
Proteolytic-induced epitope retrieval (PIER) techniques result in a degradation of peptides to unmask the epitopes of interest. Trypsin and proteinase K are enzymes typically used in PIER, which act by breaking down protein cross-links, hence unmasking the hidden antigens and increasing staining intensity and specificity of the primary antibody.