Autoantibody binding and unique enzyme-substrate intermediate …
2023年10月5日 · We demonstrate that cleaved TG3, upon binding of a substrate-mimicking inhibitor, undergoes a large conformational change as a β-sheet in the catalytic core domain moves and C1C2 detaches. The...
Enzyme clustering accelerates processing of intermediates through ...
2014年9月28日 · We present a quantitative model to demonstrate that coclustering multiple enzymes into compact agglomerates accelerates the processing of intermediates, yielding the same efficiency benefits as...
Substrate channelling as an approach to cascade reactions
2016年3月22日 · Substrate channelling, where intermediates between enzymatic steps are not in equilibrium with the bulk solution, enables increased efficiencies and yields in reaction and...
The Molecular Basis of Substrate Channeling - Journal of …
Substrate channeling is the process of direct transfer of an intermediate between the active sites of two enzymes that catalyze sequential reactions in a biosynthetic pathway (for reviews see Refs. 1 and 2). The active sites can be located either on separate domains in a multifunctional enzyme or on separate subunits in a multienzyme complex.
3D Structure of the Transient Intermediate of the Enzyme–Substrate …
2023年8月18日 · We report the solution structure of an authentic sortase A thioester intermediate, including the bound substrate. The structure was determined by a combination of site-specific tagging, selective isotope labeling, and paramagnetic nuclear magnetic resonance spectroscopy.
Substrate Channeling - an overview | ScienceDirect Topics
Substrate channeling is defined as a process in which two or more successive enzymes in a metabolic pathway interact to transfer a metabolite (or intermediate) from the active site of the earlier enzyme to the active site of the later enzyme without allowing diffusion of the metabolite into the bulk phase (Ovádi, 1991).
Covalent Enzyme-Substrate Intermediates | Science - AAAS
A literature search reveals 60 cases in which there is strong evidence for covalent enzyme-substrate intermediates. You can view the full content in the following formats: ANDERSON, B.M., REACTIVITY AND CATALYSIS IN REACTIONS OF SERINE HYDROXYL GROUP AND OF O-ACYL SERINES, JOURNAL OF BIOLOGICAL CHEMISTRY 236: 455 (1961).
Covalent enzyme-substrate intermediates in transferase reactions
1973年9月1日 · In this mechanism, a catalytic group (or atom) within the active site of the enzyme links covalently with one of the substrates, or some fragment of it, at some stage of the reaction.
Mechanisms and Effects of Substrate Channelling in Enzymatic
2021年12月29日 · Substrate or metabolite channelling is a transfer of intermediates produced by one enzyme to the sequential enzyme of a reaction cascade or metabolic pathway, without releasing them entirely into bulk.
Covalent Nucleophilic Catalysis: Structures of Enzyme …
2021年5月31日 · The chemical structure of an intermediate can define how a reaction proceeds, how the enzyme responds to binding of substrate, which residues of the active site are involved in the chemical transformation and how the substrate undergoes the rearrangements needed to produce the products.
- 某些结果已被删除