A Protocol for the Comprehensive Flow Cytometric Analysis of …
We developed a protocol for the flow cytometric analysis of non-lymphoid tissues, including methods of tissue preparation, a 10-fluorochrome panel for cell staining, and a standardized gating strategy, that allows the simultaneous identification and quantification of all major immune cell types in a variety of normal and inflamed non-lymphoid ti...
CD206+ M2-like macrophages regulate systemic glucose
2017年8月18日 · a Representative flow cytometry analysis of CD11c and CD206 expression in F4/80 + SVF cells of eWAT from WT and CD206DTR mice. Cells were also stained with isotype control antibodies for anti...
CD206 (MMR) Monoclonal Antibody (MR6F3), APC (17-2061-82)
Invitrogen Anti-CD206 (MMR) Monoclonal (MR6F3), eBioscience™, Catalog # 17-2061-82. Tested in Flow Cytometry (Flow) applications. This antibody reacts with Mouse samples. Supplied as 100 µg purified antibody (0.2 mg/mL).
BD Pharmingen™ Alexa Fluor® 647 Rat Anti-Mouse CD206 - BD …
Two-color flow cytometric contour plots showing the correlated expression of CD206 (or Ig Isotype control staining) versus CD107b were derived from gated events with the forward and side-light scattering characteristics of intact PEC. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System. For Research Use Only.
CD206+ M2-like macrophages regulate systemic glucose …
a Representative flow cytometry analysis of CD11c and CD206 expression in F4/80 + SVF cells of eWAT from WT and CD206DTR mice. Cells were also stained with isotype control antibodies for anti-CD11c and anti-CD206 antibodies (left panel).
Critical role of CD206+ macrophages in promoting a cDC1-NK …
2023年10月31日 · To highlight CD206 surface expression variation across Mono/Mac differentiation in tumors, we identified relevant subsets from previously published single cell transcriptomics in B16F10 tumors (Fig. 1A, ), and applied flow cytometry to gate on those populations in a related B78chOVA (; B78: an amelanotic clone of …
Mannose receptor (CD206) activation in tumor-associated ... - AAAS
Flow cytometry experiments with CD11b + F4/80 + Gr1 − macrophages gated on alive cells using the M1 marker CD86 and M2 marker CD206 showed rapid induction of CD86 expression with an increase in the CD86 + CD206 + double-positive macrophage fraction (87.8% versus 10.3% in vehicle-treated control) within 30 min of starting treatment with RP-182 ...
The mannose receptor (CD206) identifies a population of …
2021年10月4日 · We developed 12-parameter flow cytometry protocols to identify and human intestinal MNPs. We used these protocols to purify and characterize colonic macrophages from colonic tissue from...
CD206+ macrophages transventricularly infiltrate the early …
To more extensively investigate the fate conversion capacity of intraventricular macrophages, we experimentally transplanted GFP-labeled CD206 + macrophages, isolated by flow cytometry from the meningeal cells of the E12.5 Cx3cr1-gfp +/− mice, into the lateral ventricles of wild-type E12.5 mice (Figures 2E, 2F, and S2B).
Depletion of CD206 - Nature
2022年11月21日 · We show that depletion of CD206 + M2-like macrophages activates FAPs and activated FAPs secrete follistatin, a promyogenic factor, thereby boosting the recovery process. Conversely, deletion of...