Intrathymic Injection - PMC
Intrathymic injection is used in several T cell-associated immunological studies to deliver cells or other substances directly into the thymus. Here, we describe the intrathymic injection procedure involving surgical incision of the mouse with or without a thoracotomy.
Intrathymic Injection - PubMed
Intrathymic injection is used in several T cell-associated immunological studies to deliver cells or other substances directly into the thymus. Here, we describe the intrathymic injection procedure involving surgical incision of the mouse with or without a thoracotomy.
Intrathymic dendritic cell-biased precursors promote human T cell ...
2023年1月26日 · Here we showed that DC-biased precursors depended on the expression of the transcription factor IRF8 to express the membrane-bound precursor form of the cytokine TNF (tmTNF) to promote...
Intrathymic differentiation of natural antibody-producing plasma …
2021年10月1日 · We reveal that these plasma cells differentiate intrathymically and secrete immunoglobulins with a reactivity profile characteristic of natural antibodies. Overall, this study establishes the...
Protocol for standardized intrathymic injection in mice
2023年3月17日 · Here, we describe a standardized intrathymic injection protocol that requires only basic equipment with a minimally invasive procedure. We detail steps to identify injection sites for intrathymic delivery. We then describe how to visualize a successful intrathymic injection by including Indian ink in the injected solution.
CXXC finger protein 1 is critical for T-cell intrathymic ... - Nature
2016年5月23日 · To understand the role of Cxxc1 in intrathymic T-cell development, we generated Cxxc1 conditional knockout mouse strains and found that the intrathymic T-cell development was blocked severely in...
An Overview of the Intrathymic Intricacies of T Cell Development
2014年5月1日 · In this article, we introduce the various stages of mouse thymocyte development and highlight recent work using various in vivo, and, where appropriate, in vitro models of T cell development that led to discoveries in the regulation afforded by transcription factors and receptor–ligand signaling pathways in specifying, maintaining, and promoting...
An overview of the intrathymic intricacies of T cell development
2014年5月1日 · In this article, we introduce the various stages of mouse thymocyte development and highlight recent work using various in vivo, and, where appropriate, in vitro models of T cell development that led to discoveries in the regulation afforded by transcription factors and receptor-ligand signaling pathways in specifying, maintaining, and promoting...
Intrathymic Selection and Defects in the Thymic Epithelial Cell ...
2020年10月2日 · Intimate interactions between thymic epithelial cells (TECs) and thymocytes (T) have been repeatedly reported as essential for performing intrathymic T-cell education. Nevertheless, it has been described that animals exhibiting defects in these interactions were capable of a proper positive and negative T-cell selection.
Limiting dilution analysis of the stem cells for T cell lineage
1986年10月15日 · Stem cell activities of bone marrow, spleen, thymus, and fetal liver cells for T cell lineage were studied comparatively by transferring the cells from these organs through i.v. or intrathymus (i.t.) route into right leg- and tail-shielded (L-T-shielded) and 900 R-irradiated recipient mice, which we …