LC3 and p62 modulation after EBSS ± Baf. A1 treatment in four …
In this study, after promoting the expression of miR-224-5p, significant increases of the number of LC3 autophagic spots and LC3-II and Beclin-1 expression and a decrease of p62 expression...
P62 related autophagy and mitophagy. When autophagy happened, LC3 …
Protein p62, which is also called sequestosome 1, is continually hydrolyzed by autophagy-lysosome system after being recruited to the autophagosomes by LC3 interaction [73, 74]. Thus, p62...
搞定这 2 个明星分子 LC3、p62，看清自噬真面目（附信号通路图）
2023年9月21日 · p62 是研究广泛的一个自噬底物。在自噬体形成过程中，p62 作为连接 LC3 和聚泛素化蛋白之间的桥梁，被选择性地包裹进自噬体，之后被自噬溶酶体中的蛋白水解酶降解（图 2） [1] ，所以 p62 蛋白的表达量与自噬活性呈现负相关。 图 2. p62 介导的选择性自噬模型
自噬的检测方法（二） - 知乎 - 知乎专栏
研究表明，p62蛋白锌指结构域和uba结构域之间的连接区域（lrs区域）负责与自噬受体蛋白 atg8/lc3结合，uba结构域负责招募 泛素化 蛋白。 在自噬体形成过程中，p62作为链接LC3和聚泛素化蛋白之间的桥梁，被选择性地包裹进自噬体，之后被 自噬溶酶体 中的蛋白水解
Structural Basis for Sorting Mechanism of p62 in
2008年8月15日 · These results demonstrate that the intracellular level of p62 is tightly regulated by autophagy through the direct interaction of LC3 with p62 and reveal that selective turnover of p62 via autophagy controls inclusion body formation.
Quantification of LC3 and p62 levels in the presence or …
Microtubule-associated protein 1 (LC3) is widely used as a marker for autophagosomes and migrates as an ϳ 16 kDa “unprocessed” form, termed LC3-I, and an ϳ 16 phosphatidylethanolamine-...
LC3与P62之间的关系 - 丁香园论坛 - DXY.cn
以观察到弥散型 p62 和聚集型 p62 的分布。目前通过. 检测 p62 判断自噬流最为准确的方法是联合 Western. blot 和免疫染色技术, 包括免疫组化和免疫荧光。一. 方面可以检测到细胞内p62不同形态的含量变化, 另. 一方面可以观察不同形式 p62 在细胞内的定位。总体
LC3- and p62-based biochemical methods for the analysis of …
2015年3月15日 · Here, we outline detailed methodologies for LC3- and p62-based biochemical analysis of autophagy progression. We also discuss possible pitfalls and important cautions regarding LC3 and p62 immunoblot analysis.
Aichi virus 3C protease modulates LC3- and SQSTM1/p62 …
2020年7月14日 · Ectopic overexpression of LC3 and p62, but not Atg proteins, contributed to RLR antiviral signal axis, shRNA knockdown of LC3 and p62 led to a downregulation of antiviral inflammation. Moreover, AiV infection inhibited double-stranded RNA (dsRNA)-activated RLR activity by the viral protein 3C protease but not H42D, C143S protease dead mutants.
p62 colocalizes with fragmented mitochondria and LC3-II. (A) …
Researchers observed these changes to be associated with augmented ratio of LC3-phosphatidylethanolamine conjugates (LC3-II/LC3-I) and enhanced expression of ubiquitin-binding protein...