Identification and characterization of the CDK1-BMAL1-UHRF1 …
2023年4月21日 · Immunoprecipitation assay revealed that BMAL1 bound to CDK1, but not STK38 (Figure 2 B). Immunofluorescence analysis also confirmed the colocalization between BMAL1 and CDK1 (Figure 2 C).
CDK1 bridges NF-κB and β-catenin signaling in ... - ScienceDirect
2023年1月31日 · Using public datasets, quantitative real-time PCR, western blot, and immunohistochemical (IHC) analyses, we detect high levels of CDK1 in human and mouse gastric tumors. H. pylori infection induces activation of nuclear factor κB (NF-κB) with a significant increase in CDK1 in in vitro and in vivo models (p < 0.01).
Cyclin-dependent kinase 1-mediated phosphorylation of protein kinase …
2020年5月1日 · We identified cyclin-dependent kinase 1 (CDK1) as the corresponding kinase for PKN protein phosphorylation. CDK1 phosphorylates PKN1 at S533, S537, S562, and S916 in vitro and in cells during drug-induced mitotic arrest. Immunofluorescence staining further confirmed that PKN1 phosphorylation occurs during normal mitosis in a CDK1-dependent manner.
Cyclin B1/Cdk1 Coordinates Mitochondrial Respiration for Cell …
The mitochondrial localization of cyclin B1/Cdk1 was further studied by immunofluorescence assays (IFAs) with double staining using antibodies to cyclin B1 or Cdk1 along with antibodies to COX IV. The results of 3D deconvolution fluorescence microscopy ( Figures 1 K and 1L, upper panels) showed that cyclin B1 and Cdk1 were colocalized with COX ...
Commercial Cdk1 Antibodies Recognize the Centrosomal Protein …
2024年2月16日 · Immunofluorescence experiments showed that the Cdk1-SNAP signal resembled the staining obtained with the 610038 antibody and not the sc-54 antibody (Supplementary Figure S2E and F). To investigate whether the distribution of Cep152 and Cdk1 at centrosomes may differ, we performed STED (stimulated emission depletion) microscopy.
Quantitative differences between cyclin-dependent kinases …
2021年10月12日 · AID CDK1 KO CDK1 cells were incubated in medium without or containing DI for 24 h before being fixed and analyzed with immunofluorescence microscopy (blue: DNA; red: pericentrin; overlay with bright field). Examples of cells with metaphase plate in a control and CDK1-deficient cells are shown (scale bars: 5 μm).
Cyclin-dependent kinase 1 disruption inhibits angiogenesis by ... - PubMed
Western blotting, reverse transcription-quantitative PCR (RT-qPCR) analysis, immunofluorescence and immunohistochemistry were used to evaluate the expression of CDK1 in pathological angiogenesis using an oxygen-induced retinopathy (OIR) mouse model. Small interfering (si)RNA sequences against CDK1 were synthesized and incubated with retinal cells.
CDK1 promotes nascent DNA synthesis and induces resistance of …
2017年10月10日 · CDK1 inhibition induces a DNA damage response mainly in replicating cells. A. Representative immunofluorescence images of γH2AX foci in DMSO- or CDK1i-treated U2OS cells. EdU staining was used to label replicating cells.
Immunofluorescence Pattern of Cyclin B1 and Y15 Phosphorylated Cdk1 …
Activation of cyclin B1-cyclin-dependent kinase 1 (Cdk1), triggered by a positive feedback loop at the end of G2, is the key event that initiates mitotic entry. In metaphase, anaphase-promoting...
CDK1 plays an important role in the maintenance of ... - Nature
2014年11月6日 · Immunofluorescence analysis (with Ki67 and CDK1 antibodies) to distinguish the specific stages of the cell cycle (performed as in Becker et al. 21) indicated a high nuclear CDK1 accumulation...