Microridge-like structures anchor motile cilia
2022年4月19日 · a Scanning electron microscopy (SEM) revealed that microridges form at stage 26, and continued to increase in complexity towards stage 40, when cilia start to retract (scale bars, 1 µm).
Cilia metasurfaces for electronically programmable microfluidic ...
2022年5月25日 · We show a scanning electron microscope (SEM) image of a small region of the released cilia in Fig. 1b. Each cilia array is connected through a busbar that enables independent actuation.
A novel method for live imaging of human airway cilia using
2020年9月2日 · Determining the directionality of cilia is difficult. We developed a novel method to stain airway epithelial cilia to observe their movement without releasing ciliated cells.
Super-Resolution Microscopy and FIB-SEM Imaging Reveal …
Motile cilia are cellular beating machines that play a critical role in mucociliary clearance, cerebrospinal fluid movement, and fertility. In the airways, hundreds of motile cilia present on the surface of a multiciliated epithelia cell beat coordinately to protect the epithelium from bacteria, viruses, and harmful particulates.
Scanning electron microscopy of human islet cilia - PMC
2023年5月5日 · To enable ultrastructural characterization of islet primary cilia, we used surface topography imaging by scanning electron microscopy (SEM) to directly visualize the ciliary axoneme in human pancreatic islets.
Immuno-scanning electron microscopy of islet primary cilia
2024年5月28日 · Scanning electron microscopy (SEM) is a high-resolution technique uniquely suited for examining the native 3D conformation of surface structures, an approach that we recently used to characterize the primary cilia axoneme of human islets (Polino et al., 2023).
Primary cilia biogenesis and associated retinal ciliopathies
2021年2月1日 · Primary cilia have a distinct protein composition that can be attributed to a specialized ciliary trafficking machinery. Soluble proteins that are not associated with the membrane can enter the cilium by passive diffusion or …
2023年5月19日 · Scanning electron microscopy (SEM) is a useful technique for studying the surface morphology of membrane projections like cilia, but conventional sample preparation does not reveal the submembrane axonemal structure, which holds key implications for ciliary function.
Microvilli and cilia: surface specializations of mammalian cells
1977年1月1日 · In SEM, a mouse oviductal cell which is generating cilia has a daisy-like appearance (Figure 10.6b) (Dirksen, 1974), with longer cilia symmetrically encircling shorter cilia at the cell center. Strands of the ciliary necklace are seen before axonemal growth is complete, but more strands are added as the cilium matures.
A serotonergic axon-cilium synapse drives nuclear signaling to …
2022年9月1日 · FIB-SEM reveals axo-ciliary synapses. Focused ion beam-scanning electron microscopy (FIB-SEM) was used to reconstruct the microenvironment of CA1 neuronal primary cilia. In a pilot dataset (6 × 6 × 20 nm 3 voxel size), we reliably followed two CA1 cilia in a 20 × 20 × 15 μm 3 volume (Figure 1 A).
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