2012年4月23日 · HCoV-NL63 was detected in 28 (9.3%) of the 300 samples evaluated from November 2002 to April 2003. Twenty-two samples were positive for HCoV-NL63 by both simple and multiplex RT-PCR ().Discordant results were found for the remaining 6 samples: 3 were positive only in simple RT-PCR, and 3 were positive only in multiplex RT-PCR.