The effect of 1,2-dioleoyl-3-trimethylammonium propane (DOTAP …
2023年3月15日 · Liposomes have been reported as increasing both qualities. In this study, the development of β-ionone liposomes was initiated by adding 1,2-dioleoyl-3-trimethylammonium propane (DOTAP) to produce...
DOTAP Liposomal Transfection Reagent
DOTAP Liposomal Transfection Reagent is a liposome formulation of the cationic lipid DOTAP. Unlike other liposomal reagents, it produces highly efficient transfections both in the presence and absence of serum. It can also be successfully used for in vivo applications. Mixing DOTAP with DNA results in spontaneously formed stable complexes.
DOTAP/DOPE ratio and cell type determine transfection efficiency …
2015年10月1日 · Four liposome formulations with different 1,2-dioleoyl-3-trimethylammoniumpropane (DOTAP) to dioleoylphosphatidylethanolamine (DOPE) weight ratios were investigated, that is, weight ratios 1:0 (T1P0), 3:1 (T3P1), 1:1 (T1P1), and 1:3 (T1P3).
Optimization of DOTAP/chol Cationic Lipid Nanoparticles for …
2022年5月9日 · The objective of this study was to examine the effect of the molar ratio of DOTAP/chol, PEGylation, and lipid to mRNA ratio on mRNA transfection, and explore the applications of DOTAP/chol LNPs in pDNA and oligonucleotide transfection. Here we showed that PEGylation significantly decreased mRNA transfection efficiency of DOTAP/chol LNPs.
Molecular Mechanisms of Cationic Fusogenic Liposome …
2023年12月12日 · DOPE promotes the formation of liposome-membrane fusion intermediates. DOTAP equips the liposomes with a positive charge that promotes bacterial targeting and facilitates close membrane interactions due to charge attraction. Rh-PE is incorporated to visualize the interactions of cFLs with bacteria by using fluorescence microscopy techniques.
DP7-C-modified liposomes enhance immune responses and …
2020年12月10日 · The results showed that the modification of DOTAP liposomes with DP7-C increased the efficiency of liposome-mediated mRNA transfection into DCs. The penetrating properties of DP7-C may increase the efficiency of liposome entry into DCs and DP7-C may activate the DC uptake pathway thus increase the efficiency of DC uptake of …
DOTAP: Structure, hydration, and the counterion effect
2023年3月21日 · The cationic lipid 1,2-dioleoyl-3-trimethylammonium propane (DOTAP) is one of the original synthetic cationic lipids used for the liposomal transfection of oligonucleotides in gene therapy. The key structural element of DOTAP is its quaternary ammonium headgroup that is responsible for interactions with both nucleic acids and …
Evaluation of novel cationic gene based liposomes with …
2019年10月22日 · This study has investigated the encapsulation and transfection efficiency of cationic liposomes prepared from DOTAP and carboxymethyl-β-cyclodextrin (CD). The encapsulation efficiency of the...
DOTAP: Structure, hydration, and the counterion effect - Cell Press
2023年3月21日 · The cationic lipid 1,2-dioleoyl-3-trimethylammonium propane (DOTAP) is one of the original synthetic cationic lipids used for the liposomal transfection of oligonucleotides in gene therapy. The key structural element of DOTAP is its quaternary ammonium headgroup that is responsible for interactions with both nucleic acids and …
Engineering liposomal nanoparticles for targeted gene therapy
2017年5月15日 · The most commonly used phospholipids for the preparation of peptide-targeted liposomal gene delivery systems are DOTAP, DOPE, DSPC and DSPE alongside PEG. All of them contain two saturated or...