Flow cytometry for the development of biotechnological processes with ...
2013年1月1日 · In this review flow cytometry (FCM) exploiting the natural autofluorescence of photosynthetic pigments or in combination with fluorescent dyes is described as a rapid, accurate and sensitive method with the potential to contribute to efficient development of biotechnological processes with microalgae.
Rapid detection of neutral lipid in green microalgae by flow cytometry ...
2014年7月22日 · In this study, we combined flow cytometry with the lipid soluble dye Nile red to determine the staining efficiency of accumulated intracellular lipid under variable conditions. The staining method was modified by manipulating incubation …
Best practices in the flow cytometry of microalgae - PubMed
While flow cytometry (FCM) is a fast and reliable method particularly suitable for genome size estimation in plant and animal studies, its application to microalgae often comes with many methodological challenges due to specific issues (e.g., cell wall composition, and presence of various secondary metabolites).
Flow cytograms to differentiate a small cells, large cells, and ...
Flow cytometry and cell sorting are other physical methods that can differentiate between the heterotrophic population of bacteria and algae in the consortia based on their morphology and...
Best practices in the flow cytometry of microalgae
2021年2月21日 · While flow cytometry (FCM) is a fast and reliable method particularly suitable for genome size estimation in plant and animal studies, its application to microalgae often comes with many methodological challenges due to specific issues (e.g., cell wall composition, and presence of various secondary metabolites).
Flow Cytometry Pulse Width Data Enables Rapid and Sensitive
2014年5月15日 · Here, we utilise flow cytometry pulse width data for the estimation of cell density and biomass, using Chlorella vulgaris and Chlamydomonas reinhardtii as model algae and compare it to optical density methods.
Live/Dead Algal Analysis With the Attune NxT Flow Cytometer
Live and dead algae were analyzed with the Attune NxT Flow Cytometer and SYTOX Green Dead Cell Stain. The ability to quantitate different amounts of live and dead algal cells offers a screening tool for laboratories utilizing these types of organisms.
Artificial Intelligence-based Flow Cytometer for Real-time Algae ...
2024年1月1日 · By differentiating between species based on their unique characteristics, flow cytometry furnishes valuable insights into algae physiology, growth rates, and their adaptability to environmental variables [5]. Furthermore, flow cytometry can be utilized to quantify total bacteria numbers and biomass.
Nuclei isolation protocols for flow cytometry allowing nuclear …
2021年3月12日 · In this study the performance of six nuclei isolation protocols was compared on various problematic microalgae using FCM. The nuclei isolation methods involved osmotic bursting of cells, razor blade chopping of fresh biomass and two newly introduced protocols, razor blade chopping of desiccated biomass and bead beating.
Automated Online Flow Cytometry Advances Microalgal …
2021年3月22日 · Automated online FCM is a powerful tool for microalgal bioprocess optimization owing to its adaptability to myriad phenotypic assays and its compatibility with various cultivation systems. This allows advancing bioprocesses associated with both microalgal biomass and compound production.
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