The glycocalyx: a novel diagnostic and therapeutic target in sepsis
2019年1月17日 · The glycocalyx is a gel-like layer covering the luminal surface of vascular endothelial cells. It is comprised of membrane-attached proteoglycans, glycosaminoglycan chains, glycoproteins, and adherent plasma proteins. The glycocalyx maintains homeostasis of the vasculature, including controlling vascular permeability and microvascular tone, preventing microvascular thrombosis, and regulating ...
The glycocalyx as a permeability barrier: basic science and clinical ...
2022年9月12日 · Preclinical studies in animals and human clinical trials question whether the endothelial glycocalyx layer is a clinically important permeability barrier. Glycocalyx breakdown products in plasma mostly originate from 99.6–99.8% of the endothelial surface not involved in transendothelial passage of water and proteins. Fragment concentrations correlate poorly with in vivo imaging of glycocalyx ...
Resuscitation Fluid Choices to Preserve the Endothelial Glycocalyx
2019年3月9日 · The endothelial glycocalyx is a determinant of hydraulic conductivity. The endothelial glycocalyx is also an important determinant of hydraulic conductivity. Hydraulic conductivity (L p) is the change in filtration rate for a given change in transendothelial pressure, and can be thought of as the ease with which water passes across the vessel wall.
Glycocalyx and sepsis-induced alterations in vascular permeability ...
2015年12月1日 · Endothelial cells line the inner portion of the heart, blood vessels, and lymphatic vessels; a basal membrane of extracellular matrix lines the extraluminal side of endothelial cells. The apical side of endothelial cells is the site for the glycocalyx, which is a complex network of macromolecules, including cell-bound proteoglycans and sialoproteins. Sepsis-associated alterations of this ...
Plasma for prevention and treatment of glycocalyx degradation in …
2024年7月20日 · The endothelial glycocalyx, a gel-like layer that lines the luminal surface of blood vessels, is composed of proteoglycans, glycoproteins, and glycosaminoglycans. The endothelial glycocalyx plays an essential role in vascular homeostasis, and its degradation in trauma and sepsis can lead to microvascular dysfunction and organ injury. While there are no proven therapies for preventing or ...
The glycocalyx is a gel-like layer covering the luminal surface of vascular endothelial cells. It is comprised of membrane-attached proteoglycans, glycosaminoglycan chains, glycoproteins, and adherent plasma proteins.
Three-dimensional ultrastructure of capillary endothelial …
2017年10月23日 · Background Sugar-protein glycocalyx coats healthy endothelium, but its ultrastructure is not well described. Our aim was to determine the three-dimensional ultrastructure of capillary endothelial glycocalyx in the heart, kidney, and liver, where capillaries are, respectively, continuous, fenestrated, and sinusoidal. Methods Tissue samples were processed with lanthanum-containing alkaline ...
Fluid resuscitation should respect the endothelial glycocalyx layer ...
2014年12月23日 · Endothelial glycocalyx degradation induced by fluid overload adds to the concern of a detrimental effect of uncontrolled fluid resuscitation and the risk of unnecessary fluid infusion. As a consequence, the use of new tools for monitoring response to fluids appears promising. From that perspective, the monitoring of plasma concentration of glycocalyx degradation markers could be useful.
The endothelial glycocalyx degenerates with increasing sepsis …
2011年10月27日 · This clinical prospective study - approved by the local ethics committee - was performed to assess plasma levels of the glycocalyx components (hyaluronane, syndecan, heparan sulfate) by ELISA technique and polymorphonuclear leukocyte (PMN) function by flow cytometry in eight healthy volunteers (HV) and 37 patients who were prospectively enrolled within 24 hours of onset of signs of infection ...
Association of sublingual microcirculation parameters and …
2019年7月24日 · Background The endothelial glycocalyx (eGC) covers the luminal surface of the vascular endothelium and plays an important protective role in systemic inflammatory states and particularly in sepsis. Its breakdown leads to capillary leak and organ dysfunction. Moreover, sepsis-induced alterations of sublingual microcirculation are associated with a worse clinical outcome. The present study was ...