Isocitrate lyase - Wikipedia
Isocitrate lyase (EC 4.1.3.1), or ICL, is an enzyme in the glyoxylate cycle that catalyzes the cleavage of isocitrate to succinate and glyoxylate. [2] [3] Together with malate synthase, it bypasses the two decarboxylation steps of the tricarboxylic acid cycle (TCA cycle) and is used by bacteria, fungi, and plants. [4]
Crystal Structure and Molecular Mechanism of Isocitrate Lyase …
2024年10月2日 · This study presents the crystal structures of C. aurantiacus-derived ICL (CaICL), in its Mg 2+-bound and Mn 2+ and isocitrate-bound forms, elucidating its substrate-binding mechanism and catalytic loop dynamics. CaICL forms a homotetramer and interacts with isocitrate via critical active-site residues, revealing its catalytic mechanism. The ...
Mechanism-based inactivator of isocitrate lyases 1 and 2 from ... - PNAS
2017年7月5日 · Isocitrate lyase (ICL, types 1 and 2) is the first enzyme of the glyoxylate shunt, an essential pathway for Mycobacterium tuberculosis (Mtb) during the persistent phase of human TB infection. Here, we report 2-vinyl-d-isocitrate (2-VIC) as …
Isocitrate Lyase - an overview | ScienceDirect Topics
ICLase (threo-D S-isocitrate-glyoxylate lyase; EC 4.1.3.1) is the first key enzyme of the glyoxylate cycle and catalyzes the reversible aldol cleavage of isocitrate to glyoxylate and succinate. ICLase activity is usually estimated spectrophotometrically by measuring the amount of glyoxylate formed, or consumed, in an either chemically or ...
M-CSA Mechanism and Catalytic Site Atlas - EMBL-EBI
Isocitrate lyase catalyses the reversible lysis of a C-C bond of isocitrate to form glycoxylate and succinate. The enzyme directs acetyl-CoA away from beta-oxidation of fatty acids into the glyoxylate shunt pathway, giving a net carbon gain. It plays a key role in the persistence of Mycobacterium tuberculosis within humans.
Isocitrate Lyase - an overview | ScienceDirect Topics
Isocitrate lyase (ICL) is an enzyme that catalyzes the first step in the glyoxylate shunt, a pathway that allows the net synthesis of C4 dicarboxylic acids from C2 compounds such as acetate. AI generated definition based on: Current Opinion in Pharmacology, 2006
Crystal structure and functional analysis of isocitrate lyases …
2016年6月1日 · Isocitrate lyase (ICL) catalyzes the first-step reaction of the glyoxylate cycle, using isocitrate from the TCA cycle as the substrate to produce succinate and glyoxylate. In this study we report the crystal structure of Magnaporthe oryzae ICL in both the ligand-free form and as a complex with Mg 2+ , glyoxylate, and glycerol, as well as the ...
Demystifying the catalytic pathway of Mycobacterium tuberculosis ...
2020年11月3日 · As one of the key targets during the latent/dormant stage of M. tuberculosis, isocitrate lyase (ICL) has been a subject of interest for new tuberculosis therapeutics. In this work, the cleavage...
Heterogeneous multimeric structure of isocitrate lyase in …
2021年5月5日 · During the glyoxylate cycle, isocitrate lyases (ICLs) catalyze the lysis of isocitrate to glyoxylate and succinate. Itaconate has been reported to inhibit an ICL from Mycobacterium tuberculosis (tbICL). To elucidate the molecular mechanism of ICL inhibition, we determined the crystal structure of tbICL in complex with itaconate.
Probing the Catalytic Mechanism Involved in the Isocitrate Lyase ...
2015年7月29日 · The isocitrate lyase (ICL) superfamily catalyzes the cleavage of the C(2)–C(3) bond of various α-hydroxy acid substrates. Members of the family are found in bacteria, fungi, and plants and include ICL itself, oxaloacetate hydrolase (OAH), 2-methylisocitrate lyase (MICL), and (2R,3S)-dimethylmalate lyase (DMML) among others. ICL and related ...
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