LC3 lipidation is essential for TFEB activation during the lysosomal ...
2020年9月28日 · Here we show that transcription factor EB (TFEB)—a master transcriptional regulator of lysosomal biogenesis and autophagy—is activated during the lysosomal damage response, and its activation is...
LC3 lipidation is essential for TFEB activation during the lysosomal ...
Here we show that transcription factor EB (TFEB)-a master transcriptional regulator of lysosomal biogenesis and autophagy-is activated during the lysosomal damage response, and its activation is dependent on the function of the ATG conjugation system, which mediates LC3 lipidation.
Reconstitution of cargo-induced LC3 lipidation in mammalian
2021年4月23日 · The attachment of the ATG8 proteins of the LC3 and GABARAP subfamilies to the membrane lipid phosphatidylethanolamine (PE), termed LC3 lipidation, is a hallmark of autophagosome biogenesis. LC3 lipidation occurs via a ubiquitin-like conjugation cascade.
Beyond autophagy: LC3-associated phagocytosis and endocytosis
2022年10月26日 · LC3 lipidation aids in autophagosome trafficking and directs autophagosome/lysosome fusion events as well as other functions including cargo selection in canonical autophagy. In LAP, LC3 lipidation occurs on the outer face of the phagosome membrane following full phagosome closure.
Molecular mechanisms and functions of LC3 lipidation - SciEngine
Lipidation of LC3 (including all LC3/GABARAP family proteins) is a key event of autophagy process. LC3 obtains membrane-bound capacity via conjugation with phosphatidyl ethanolamine under the assistance of two ubiquitination-like systems.
Robust LC3B lipidation analysis by precisely adjusting ... - Nature
2022年1月7日 · Autophagic flux can be quantified based on the accumulation of lipidated LC3B in the presence of late-stage autophagy inhibitors. This method has been widely applied to identify novel compounds...
Lipidation of the LC3/GABARAP family of autophagy proteins …
Here we demonstrate that the E2-like enzyme, Atg3, facilitates LC3/GABARAPlipidation only on membranes exhibiting local lipid-packing defects. This activity requires an amino-terminal amphipathic helix similar to motifs found on proteins targeting highly …
LC3B is lipidated to large lipid droplets during prolonged …
2023年7月24日 · Omrane and Ben M’Barek et al. found that long-term starvation triggers a noncanonical autophagic process on lipid droplets (LDs). This process involves the binding of Atg3 to specific, ultra-large micrometric LDs, lipidation of LC3B to these LDs, and recruitment of acidified autophagosome-like membranes for degradation.
Lipidation of the LC3/GABARAP family of autophagy proteins
2014年4月20日 · Here we demonstrate that LC3/GABARAP lipidation requires local membrane stress associated with lipid-packing defects as occurs with high molar proportions of conical lipids or strident...
Regulation of LC3 lipidation by the autophagy-specific class III ...
2019年4月11日 · We used a cell-free reaction that reproduces a key autophagy initiation step, LC3 lipidation, as a biochemical readout to probe the role of autophagy-related gene (ATG)14, a PI3KC3-C1-specific subunit implicated in targeting the complex to autophagy initiation sites.