The Organic Anion Transporter (OAT) Family: A Systems …
Oat1 and Oat3 (A), localized to the basolateral membrane of the proximal tubule cell, transport OA − across the basolateral membrane and into the cell through the exchange of dicarboxylates (DC −).
Organic anion transporter 1 - Wikipedia
OAT1 is a transmembrane protein that is expressed in the brain, the placenta, the eyes, smooth muscles, and the basolateral membrane of proximal tubular cells of the kidneys. It plays a central role in renal organic anion transport.
OAT1/3在肾小管表达差异及竞争性抑制对急性马兜铃酸Ⅰ肾小管 …
2024年10月12日 · Hematoxylin-eosin (HE) staining was employed to examine the pathological alterations in renal tissue. Immunohistochemical (IHC)staining was conducted to assess the tissue localization and protein expression levels of OAT1 and OAT3 in renal tubules.
Immunostaining of OAT1 in the kidney cortex of prepubertal male …
OAT1 staining (red fl uorescence) in proximal tubules of male ( A ) and female ( B ) kidneys was weak and similar in intensity. Green fl uorescence represents the staining of megalin.
Immunohistochemical studies of organic anion transporters and …
2012年5月8日 · Various substances including uric acid, organic acids and drugs are transported by organic anion transporters (OATs) in the kidney. In addition, a member of the OAT family, urate transporter 1 (URAT1), is involved in the reabsorption of uric acid from the renal tubule. Benzbromarone inhibits URAT1 to block uric acid reabsorption. Methods.
Developmental expression of renal organic anion transporters in …
2012年6月15日 · Organic anion transporters (OAT1 and OAT3) and multidrug resistance-associated proteins (MRP2 and MRP4) play important roles in anionic drug secretion in renal proximal tubules. Changes in the expression of such transporters are considered to affect the tubular secretion of anionic drugs.
Sex-dependent expression of Oat3 (Slc22a8) and Oat1 (Slc22a6) …
2013年4月4日 · To perform double staining of mOat3 and actin, and of mOat1 and actin, the kidney cryosections were first stained for Oat3 or Oat1 proteins as described above and then incubated with the actin-Ab (1:50) at 4°C overnight, washed, and incubated with GAMF (1:100) at room temperature for 60 min.
Developmental changes in multispecific organic anion transporter 1 ...
2000年4月1日 · In both the fetal and neonatal kidneys, OAT1 mRNA was localized in a relatively deep region in the cortex. Western blot analysis detected OAT1 protein on embryonic day 20, and the expression level increased after birth. Immunohistochemical analysis did not reveal OAT1 staining in the fetal kidneys.
A Simple and Rapid Bioluminescence-Based Functional Assay of
Organic anion transporter 1 (SLC22A6/OAT1) plays a key role in renal tubular excretion of endo- and exogenous anionic substances including drugs. Since the inhibition of OAT1 function by a concomitant drug may cause pharmacokinetic drug-drug interactions (DDIs) in …
Folic acid reverses uric acid crystal-induced surface OAT1 ...
Surface and internalization OAT1 staining. OAT1 stable HEK cells were treated with or without MSU for 24 h and live cells were used for staining the OAT1 extracellular region at 4°C for 1 h. Cells were then transferred back to the 37°C incubator for 30 min to allow for internalization.
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