HaloTag® Technology: A Powerful Tool for Protein ... - Promega …
Explore how to analyze proteins with HaloTag® Technology. Start with a single protein fusion with HaloTag® protein and you can purify proteins, investigate protein:protein and protein:DNA interactions, image cells and more.
HaloTag蛋白标签技术_专题_Promega微网站-普洛麦格（北京）生 …
该技术基于HaloTag®蛋白和合成配基之间形成的共价键，这些配基具有多种功能，包括荧光标记、亲和标记和附着在固相载体上；而共价键在生理条件下可迅速形成，且具有高度特异性和本质上的不可逆，形成的复合物即使在变性条件下也是稳定的。 因此，使用HaloTag®只需要一个可以表达的单一融合结构，用任何一种荧光分子标记，即可在活细胞或固定细胞中进行成像。 了解更多HaloTag®技术. 使用HaloTag®融合蛋白和HaloTag®荧光配基进行细胞成像。 您可以对洗涤 …
Janelia Fluor®HaloTag®Ligands_产品_Promega微网站-普洛麦 …
这些Janelia Fluor® HaloTag®染料涵盖了整个可见光谱范围，凭借其独特的荧光生成特性与多用途性能及细胞通透性，使其成为标记细胞内蛋白质的理想选择。 JFX染料（Janelia Fluor®染料的类似物）在罗丹明类化合物的烷基氨基取代基中引入氘原子，从而抑制了光化学诱导的光谱漂移，并降低了不可逆的光漂白现象。 Janelia Fluor®和JFX染料因其卓越的光稳定性，使其在活细胞中进行检测及单分子成像研究时具有无可估量的价值。 这些染料已应用于多种成像方法，包括针 …
HaloTag® Technology: Novel New Applications - Promega
HaloTag® protein attached to protein of interest (POI), combines with chloroalkane ligand and functional group via a covalent bond. Figure 2. Schematic of mammalian protein purification using HaloTag® technology. Canine mammary tumours are …
HaloTag® Technology: A Powerful Tool for Protein Labeling ... - Promega
Explore how to analyze proteins with HaloTag® Technology. Start with a single protein fusion with HaloTag® protein and you can purify proteins, investigate protein:protein and protein:DNA interactions, image cells and more.
HaloTag Vectors for Protein Analysis - Promega
HaloTag® technology is a comprehensive platform for functional protein analysis based on the formation of a covalent bond between a protein fusion tag (HaloTag) and synthetic chemical ligands. By using different ligands you can control the …
Magne™ HaloTag®Beads_产品_Promega微网站-普洛麦格（北 …
Magne™ HaloTag ® Beads 提供了一个共价捕获 HaloTag ® 融合蛋白的简便方法，它应用磁性颗粒进行蛋白 pull-down 和纯化。 这些磁珠对 HaloTag ® 融合蛋白具有高结合能力 ( ≥ 20mg/ml )，而对非特异蛋白的结合能力低。 磁性处理的特点简化了蛋白纯化过程，免除了多个离心步骤，并促进在机器人平台上的自动化应用。 推荐使用 Magne™ HaloTag ® Beads ( Cat.# G7281 和 G7282 ) 替代已停产产品 HaloLink™ Magnetic Beads ( Cat.# G9311 )。 Promega公司是为生 …
Using HaloTag® Technology to Image Advanced Cell Models - Promega
HaloTag ® Technology delivers a groundbreaking approach to protein labeling, providing researchers with a powerful tool to study protein dynamics with unparalleled precision and flexibility.
HaloTagTM Interchangeable Labeling Technology(a–c) is useful for imaging, capturing or immobilizing fusion proteins. The HaloTagTM Protein binds rapidly and specifically to chloro-alkane ligands modified to carry a variety of functional chemical tags. The nature of the available ligands facilitates imaging at different wavelengths.
HaloTag® Fluorescent Ligands - Promega
HaloTag® Fluorescent Ligands enable you to add the chloroalkane group, with which HaloTag® protein reacts, to any compound or surface with a compatible chemical group. The possible applications are endless. Pull Down Protein Complexes: The spacer and reactive linker of the HaloTag® PEG-Biotin Ligand provide ideal pull-down capabilities.