Synthetic Chemical Inducers and Genetic Decoupling Enable …
The introduction of a stem loop into the translation initiation region of the rhaPBAD promoter resulted in the most significant improvement of eGFP expression. Starting from this plasmid, we constructed a set of expression vectors bearing different genetic modules like rop, ccdAB, cer and combinations thereof, and tested the efficiency of ...
pCK306 - Addgene
rhaBAD, a rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites. Allows precise & sustained gene expression in cyanobacteria.
The Escherichia coli rhaSR-PrhaBAD Inducible Promoter System …
The rhaBAD promoter (AGGTCG-17-TAGACT) is weak in E. coli. Activation of the rhaBAD promoter occurs through a regulatory cascade involving RhaR and RhaS . RhaR is a transcription factor that binds rhamnose and activates the transcription of …
Part:BBa K914003 - parts.igem.org
PRha (the rhamnose promoter) is an inducible promoter commonly used for gene expression regulation in Escherichia coli. It is activated in the presence of L-rhamnose, driving the transcription of downstream genes with high controllability.
The Escherichia coli rhamnose promoter rhaP BAD is in …
2009年9月30日 · We developed an expression vector system based on the broad host range plasmid pBBR1MCS2 with the Escherichia coli rhamnose-inducible expression system for applications in Pseudomonas. For validation and comparison to E. coli, enhanced green fluorescent protein (eGFP) was used as a reporter.
Optimization of an E. coli L-rhamnose-inducible expression …
2008年1月14日 · The introduction of a stem loop into the translation initiation region of the rhaP BAD promoter resulted in the most significant improvement of eGFP expression. Starting from this plasmid, we constructed a set of expression vectors bearing different genetic modules like rop, ccdAB, cer and combinations thereof, and tested the efficiency of ...
The Escherichia coli rhaSR-PrhaBAD Inducible Promoter System
The Escherichia coli rhaSR-PrhaBAD Inducible Promoter System Allows Tightly Controlled Gene Expression over a Wide Range in Pseudomonas aeruginosa. Meisner J, Goldberg JB Appl Environ Microbiol. 2016 Oct 27;82(22):6715-6727. doi: 10.1128/AEM.02041-16.
The Escherichia coli rhaSR-PrhaBAD Inducible Promoter System Allows ...
2016年10月27日 · The araC-ParaBAD inducible promoter system is tightly controlled and allows gene expression to be modulated over a wide range in Escherichia coli, which has led to its widespread use in other bacteria. Although anecdotal evidence suggests that araC-ParaBAD is leaky in Pseudomonas aeruginosa, neither …
Synthetic Chemical Inducers and Genetic Decoupling Enable
2016年10月21日 · External control of gene expression is crucial in synthetic biology and biotechnology research and applications, and is commonly achieved using inducible promoter systems. The E. coli rhamnose-inducible rhaBAD promoter has properties superior to more commonly used inducible expression systems, but i …
rhamnose promoter for gene expression in P. putida KT2440 requires the additional expression of both activa-tors, RhaR and RhaS. Therefore, we constructed expression vectors harboring rhaR, rhaS, and the reporter enhanced green fluorescent protein (eGFP) controlled by the rhaP BAD promoter. The evaluation of the strain and plasmid