Elucidation of the core betalain biosynthesis pathway in …
2021年3月17日 · To reconstruct the core betalain biosynthesis pathway of A. tricolor, AmCYP76AD1, AmDODAα1, and AmcDOPA5GT were transiently overexpressed in N. benthamiana leaves by agroinfiltration for the ...
Enzyme and Pathway Engineering for Improved Betanin …
2024年6月11日 · Here, we performed combinatorial engineering of plant P450 enzymes and precursor metabolisms to improve the de novo production of betanin in Saccharomyces cerevisiae. Semirational design by computer simulation and molecular docking was used to improve the catalytic activity of CYP76AD.
Casting light on the pathway to betalain biosynthesis: A review
2021年6月1日 · Summary of the main regulatory pathways of betalain biosynthesis in response to light cues and some key interacting factors. To facilitate interpretation, the putative differential mode of action of members from the family of blue (B) and red (R) light photoreceptors was not discriminated (see text for details).
Synthesis of betanin by expression of the core betalain …
2024年5月1日 · Based on the elucidation of its core biosynthetic pathway, betalain can be produced in additional plants by metabolic engineering. Synthesis of betalain in carrot (Daucus carota L.) can improve its nutritional quality and economic value by extracting betalain from the fleshy root, non-edible part, and processing residue of carrot.
Beet red food colourant can be produced more sustainably with
2023年11月29日 · Completing the betanin biosynthesis pathway with BvSGT2 resulted in ~9.9 mg l −1 betacyanins (8.6 mg l −1 betanin, 1.3 mg l −1 isobetanin), nearly threefold more than with DbB5GT ...
Combinatorial engineering of betalain biosynthesis pathway in …
2023年8月17日 · The highest titer of betanin (30.8 ± 0.14 mg/L after 48 h from 20 g/L glucose) was obtained when completing the biosynthesis pathway with UGT73A36 glucosyltransferase from Beta vulgaris. Finally, we investigated betalain transport in CEN.PK and S288C strains of Saccharomyces cerevisiae and identified a possible role of transporter genes QDR2 ...
Engineering Betalain Biosynthesis in Tomato for High Level Betanin ...
2021年6月8日 · The red pigment from red beets, betanin, is made from tyrosine by a biosynthetic pathway that consists of a cytochrome P450, a L-DOPA dioxygenase, and a glucosyltransferase. The entire pathway was recently reconstituted in plants that do not make betalains naturally including potato and tomato plants.
Biosynthesis of betalains: yellow and violet plant pigments
2013年6月1日 · Betalains are the yellow and violet pigments that substitute anthocyanins in plants belonging to the order Caryophyllales. These pigments have attracted much attention because of their bioactivities, which range from an antioxidant capacity to the chemoprevention of cancer.
Elucidation of the first committed step in betalain biosynthesis ...
2015年12月18日 · The first committed step in the pathway, tyrosine 3-hydroxylation, is catalyzed by both cytochrome P450s CYP76AD1 and CYP76AD6 in red beet. Dashed lines designate reactions of an alternative pathway for formation of betanin, where cyclo-DOPA is first glycosylated and then condensates with betalamic acid. …
Advances and future directions in betalain metabolic engineering
2019年5月30日 · The betalain biosynthetic pathway (Fig. 1) holds several features that make it particularly amenable to metabolic engineering: it involves only a few enzymatic steps, alongside spontaneously occurring cyclization and conjugation reactions, the latter offering great versatility with regard to the possible end-products that can be formed and the ...
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