Cytotoxic T Cell Overview | Thermo Fisher Scientific - US
It provides a convenient flow cytometry–based assay for detection of effector cells killer activity. In this cytotoxicity assay, effector cells can be labelled with PKH26 or antibody specific to CD8 + and cultured with target cells. Apoptosis of target cells can be detected by staining with FITC or PE conjugates of annexin V. Furthermore ...
The cytotoxic T lymphocyte assay for evaluating cell-mediated …
Among measures of CMI, the cytotoxic T Lymphocyte (CTL) response is recognized as perhaps the most relevant functional measure that reflects cell-mediated acquired immune defense against viral infections and cancer. The CTL response against T-dependent antigens requires the cooperation of at least three different major categories of immune cells.
细胞毒实验技术-细胞毒性 T 细胞功能测定-丁香实验
2022年2月10日 · 细胞毒性 T 细胞功能测定的基本原理是细胞毒性 T 细胞（CTL）是特异性细胞免疫的主要效应细胞之一，一般指 CD8＋T 细胞，可特异而高效地杀伤靶细胞，参与抗病毒免疫、抗肿瘤免疫和移植排斥反应。 它对靶细胞的杀伤首先基于对靶细胞表面特异性抗原的识别，CTL 可以通过其 TCR 识别靶细胞表面的抗原肽-MHC 复合物，其中抗原肽可来自肿瘤抗原、病毒抗原或自身抗原。 识别后，CTL 通过脱颗粒和直接接触杀伤靶细胞，外周血淋巴细胞包含针对不同抗 …
The Cytotoxic T Lymphocyte Assay for Evaluating Cell ... - Springer
2009年10月30日 · Among measures of CMI, the cytotoxic T Lymphocyte (CTL) response is recognized as perhaps the most relevant functional measure that reflects cell-mediated acquired immune defense against viral infections and cancer. The CTL response against T-dependent antigens requires the cooperation of at least three different major categories of immune cells.
CTL 杀伤实验 不懂操作步骤的同学看这里 - biomart.cn
2015年7月9日 · CTL 杀伤靶细胞主要有两种途径：即细胞裂解性杀伤和诱导细胞凋亡 ( Apoptosis)。 前者指 CTL 分泌诸如穿孔素一类的介质损伤靶细胞膜； 后者指 CTL 通过表面 FasL 与靶细胞表面的 Fas 结合，或者通过释放粒酶 B 至靶细胞后诱导靶细胞凋亡 (Apoptosis)。
In vivo assessment of specific cytotoxic T lymphocyte killing
2013年6月1日 · We detail the procedure and analysis of the in vivo CTL assay using a model of genetic immunization using as vector human recombinant replication-deficient adenovirus 5 (HAd5) containing different transgenes and discuss the use and limitations of this test.
Induction and Measurement of Cytotoxic T Lymphocyte Activity
2018年2月21日 · In this unit, several assays are described that are commonly utilized to induce CTLs and to measure CTL activity both in vitro and in vivo. These assays are adaptable to many experimental and/or disease models, and in the case of the in vitro assays can be applied to measure CTL activity in human samples. © 2018 by John Wiley & Sons, Inc.
Induction and measurement of cytotoxic T lymphocyte activity
2023年3月11日 · Cytotoxic T lymphocytes (CTL) kill target cells on the basis of cell-surface antigen recognition and are important in the host response to tumors, transplants, and viruses. This unit presents several protocols for generating and measuring CTL activity.
ELISPOT Assay for Monitoring Cytotoxic T Lymphocytes (CTL
2012年5月10日 · Assays that can detect both cytotoxic T lymphocytes (CTL) frequency and function, such as the IFN-γ enzyme-linked immunospot assay (ELISPOT) have gained increasing popularity for monitoring clinical trials and in basic research.
Cytotoxic T-Cells: Methods and Protocols | SpringerLink
Beginning with phenotypical characterization of CTL populations, the volume continues with in vitro and in vivo cytotoxicity assays, methods to detect senescent T cells, in vivo and in vitro models to understand immune and bone cells cross-talk, microscopy and in vivo imaging, as well as “Omics” approaches and molecular methods, concluding ...