LAB 5: DIRECT STAIN AND INDIRECT STAIN - Community …
3. State in chemical and physical terms the principle behind direct staining and the principle behind indirect staining. B. DIRECT STAINING . PROCEDURE . 1. Transfer a small number of bacteria from an agar surface or a broth culture to a glass slide and fix the preparation to the slide. 2. Prepare a direct stain when given all the necessary ...
Direct Stain: - University of Wyoming
Direct Stain: The cell wall of most bacteria has an overall net negative charge and thus can be stained directly with a single basic (positively charged) stain or dye. This type of stain allows us to observe the shape, size and arrangement of bacteria. Use the smear prepared in the previous procedure. Staining is done at the sink.
5: Direct Stain and Indirect Stain - Biology LibreTexts
5.2: Procedure for slide preparation prior to staining; 5.3: Procedure for Direct Staining Using a Basic Dye; 5.4: Procedure for Indirect Staining Using an Acidic Dye; 5.5: Performance Objectives for Lab 5; 5.6: Self Quiz; 5.7: Concept Map
5.3: Procedure for Direct Staining Using a Basic Dye
In direct staining the positively charged color portion of the basic dye combines with the negatively charged bacterium and the organism becomes directly stained. Learning Objectives. Videos reviewing techniques used in this lab: How to Heat-Fix a Microscope Slide;
Jennifer Trice Lab 4 Bacterial Morphology and Staining …
3. Negative staining results in a colorless cell on a stained background. True False Photo 1: Mouth - Direct Stain Slide Crystal violet is a basic stain. Congo red is an acidic substance. I was able to come to this conclusion because crystal violet was used in the direct stain. Only basic dyes are used in direct stains, which only color the ...
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Direct Stain and Indirect Stain | Microbiology - Biocyclopedia
Direct Staining Procedure. Transfer a small number of bacteria from an agar surface or a broth culture to a glass slide and heat-fix the preparation. Prepare a direct stain when given all the necessary materials. Results Recognize a direct stain preparation when it is observed through a microscope, and describe the shape and arrangement of the ...
Bacterial Morphology and staining technique - Studocu
Photo 1: Mouth - Direct Stain Slide ##### Het fixing is used to ensure the bacteria has been killed on the slide so the bacteria does not spread allowing for the smear to adhere to ##### the slide and the sample to take up the stain. During negative staining heat will damage certain cellular fixtures including bacterial
Experiment 2A Direct Stains | Lab02 | Virtual Edge| General ...
Direct Stains. Background. The cell wall of most bacteria has an overall net negative charge and thus can be stained directly with a single basic (positively charged) stain or dye. This type of stain allows us to observe the shape, size and arrangement of bacteria. Procedure. 1. Use the smear prepared in the previous procedure. ...
5.5: Performance Objectives for Lab 5 - Biology ... - Biology …
State in chemical and physical terms the principle behind direct staining and the principle behind indirect staining. B. DIRECT STAINING. PROCEDURE. 1. Transfer a small number of bacteria from an agar surface or a broth culture to a glass slide and fix the preparation to the slide. 2. Prepare a direct stain when given all the necessary materials.
When a staining procedure colors the cells present in a preparation, but leaves the background colorless (appearing as white), it is called a direct stain. If a procedure colors the background, leaving the cells colorless (white) it is called an indirect or negative stain. Both direct and indirect stains will be used in