Production of isotope-labeled proteins in insect cells for NMR
2018年4月23日 · This article provides an introduction into the insect cell/baculovirus expression system and its use for the production of recombinant isotope-labeled proteins. We discuss …
Stable isotopic labeling of proteins for quantitative proteomic ...
2008年12月22日 · There are two approaches for introducing a stable isotope into a protein without chemically modifying that protein, metabolic labeling using whole cells grown in …
Isotope Labeling - an overview | ScienceDirect Topics
Isotope labeling involves recombinant protein production in growth media that provide isotope-substituted metabolic precursors (15 N-ammonium chloride, 13 C-glucose).
A rapid and robust method for selective isotope labeling of proteins
We present examples in which proteins are selectively labeled with 13 C- and 15 N-amino acids and studied using magic-angle spinning solid-state NMR and pulsed EPR, demonstrating the …
Production of isotope-labeled proteins in insect cells for NMR
This article provides an introduction into the insect cell/baculovirus expression system and its use for the production of recombinant isotope-labeled proteins. We discuss recent advances in …
Isotope Labeled Protein Production - Creative Biostructure
Creative Biostructure is pleased to offer a custom stable isotope labeling service for proteins with expression and purification, as well as for nucleic acids. We specialize in labeling proteins …
Production of isotopically labeled heterologous proteins in non-
2009年9月29日 · In this paper, we review the recent advances in expression systems for isotopically labeled heterologous proteins, utilizing non- E. coli prokaryotic and eukaryotic …
Production and Use of Stable Isotope-Labeled Proteins for …
2011年1月1日 · Here, we describe the production of a stable isotope-labeled protein (PSAQ standard) using cell-free extracts, an expression strategy also known as in vitro translation.
introduction into the insect cell/baculovirus expression system and its use for the production of recombinant isotope-labeled proteins. We discuss recent advances in inexpensive isotope …
Production and use of stable isotope-labeled proteins for …
In this chapter, we describe the production of full-length stable isotope-labeled proteins (PSAQ standards) using cell-free expression devices. The purification and quality control of protein …