Optimal cutting temperature (OCT) is a compound especially good for preserving cellular ultrastructure (histology) and likely nucleic acids as well. It is also used for specialized applications where formalin fixed tissue is not optimal. This standard operating procedure (SOP) describes how tissues are frozen in OCT.
一种对包埋在最佳切割温度化合物中的组织进行鞘脂分析的简单方 …
2020年4月27日 · 我们开发并验证了一种简单且廉价的方法，可以从 OCT 化合物包埋的组织中去除 OCT 化合物。 我们的结果表明，从冷冻保存的组织中去除 OCT 化合物不会显着影响 LC-ESI-MS/MS 鞘脂测量的准确性。 我们使用经过验证的方法来分析肺癌患者的肿瘤中鞘脂与正常邻近未受累肺组织相比的变化，并确定 OCT 化合物冷冻保存的正常肺组织中鞘脂的长期稳定性。 我们发现肺癌肿瘤显着改变了鞘脂谱，并且鞘脂在 OCT 化合物冷冻保存的正常肺组织中可稳定长达 …
OCT包埋剂 - 百度百科
OCT包埋剂英文全称：optimal cutting temperature compound，是一种聚乙二醇和聚乙烯醇的水溶性混合物，已广泛用于免疫组化实验室中，其用途是在冰冻切片时支撑组织，以增加组织的连续性，减少皱折及碎裂。
Be very careful to orient the sample because it is important for the demonstration of proper morphology. 4) Carefully drop more OCT onto the specimen until it is completely covered. None of the tissue should remain exposed. 5) Try to avoid the formation of air bubbles. Remove any bubbles inside the OCT. This is
Quantitative Proteomic Analysis of Optimal Cutting Temperature …
Comparisons of protein recovery between frozen versus OCT-embedded tissue showed excellent consistency with more than 9200 proteins identified. Using an isobaric labeling strategy, we quantified more than 5400 proteins in tumor versus normal OCT …
To resolve the matter and collect better quality of tis-sue samples, we considered various conditions including freezing temperature and OCT compound using or not for cryosection of brain tissue. This research will show how each factors affect to tissue quality, contrary to common belief. Table 1. Conditions for cryosection of brain tissues.
Quantitative proteomic analysis of optimal cutting temperature …
Here we present a simple workflow for whole proteome analysis of OCT-embedded NSCLC tissue samples, which involves a simple trichloroacetic acid precipitation step. Comparisons of protein recovery between frozen versus OCT embedded tissue showed excellent consistency with more than 9,200 proteins identified.
用于质谱分析的最佳切割温度化合物中嵌入的人组织制备
在本报告中，我们概述了分析嵌入OCT的人组织标本中鞘脂的步骤和工作流程，包括洗涤组织，称量组织以进行数据归一化，脂质提取，制备用于液相色谱电喷雾电离串联质谱（LC-ESI-MS / MS）分析的样品，质谱数据集成，数据归一化和数据分析。 鞘脂是生物活性代谢物，以其在人体代谢 和疾病中的作用 而闻名1，2。 它们调节复杂的细胞过程，如细胞迁移、细胞存活和死亡、细胞运动、囊泡运输、细胞侵袭和转移、血管生成以及细胞因子的产生 1， 2， 3， 4， 5， 6， …
IHC-Frozen protocols - Abcam
2023年5月10日 · Orient the tissue as desired. Freeze the tissue using the isopentane bath. Hold the tissue mold over the isopentane bath with forceps for 10 – 20 seconds until the tissue block turns opaque. Avoid getting any isopentane on the OCT compound. Store the frozen tissue at -80°C until ready for sectioning.
Most frozen tissue samples used for biomedical research are collected and embedded in O.C.T. (Optimal Cutting Temperature) compound. O.C.T. has been known to cause ion suppression and decrease spectrum quality from MS experiments (Schwartz, Reyzer & Caprioli 2003).