Protein splicing - Wikipedia
Protein splicing is an intramolecular reaction of a particular protein in which an internal protein segment (called an intein) is removed from a precursor protein with a ligation of C-terminal and N-terminal external proteins (called exteins) on both sides.
Protein Splicing: From the Foundations to the Development of ...
2020年3月7日 · Protein splicing is a posttranslational autoprocessing event that involves the precise removal of an internal protein sequence, named intein, from a precursor protein with the concurrent ligation through a peptide bond of the flanking polypeptide sequences, which are …
Protein Splicing of Inteins: A Powerful Tool in Synthetic Biology
2022年2月21日 · Inteins are protein segments that are capable of ligating the flanking exteins (external proteins) into a new protein, a process known as protein splicing (Belfort et al., 2006). They are found in many natural organisms, such as bacteria, fungi and lower plants, and are usually embedded within essential proteins ( Belfort et al., 2006 ).
Protein splicing: how inteins escape from precursor proteins
2014年5月23日 · Inteins are nature's escape artists; they facilitate their excision from flanking polypeptides (exteins) concomitant with extein ligation to produce a mature host protein. Splicing requires sequential nucleophilic displacement reactions catalyzed by strategies similar to proteases and asparagine lyases.
Recent advances in in vivo applications of intein-mediated protein splicing
2014年2月4日 · We review recent advances in intein-mediated protein expression and modification, post-translational processing and labeling, protein regulation by conditional protein splicing, biosensors, and expression of trans-genes.
Protein Splicing: From the Foundations to the Development of ...
Expressed protein ligation is a simple and powerful method in protein engineering to introduce sequences of unnatural amino acids, posttranslational modifications, and biophysical probes into proteins of any size.
Biological Applications of Protein Splicing - ScienceDirect
2010年10月15日 · Protein splicing is a naturally occurring process in which a protein editor, called an intein, performs a molecular disappearing act by cutting itself out of a host protein in a traceless manner. In the two decades since its discovery, protein splicing has been harnessed for the development of several protein-engineering methods.
Reflections on protein splicing: structures, functions and …
Protein splicing is a novel biological reaction that implements the editing of genetic information transfer at a protein level and proceeds autocatalytically in a single molecule of precursor polypeptides.
Protein Splicing: How Inteins Escape from Precursor Proteins
Protein splicing results in ligation of the N-extein (E N) and C-extein (E C), as directed by the intein (I). When inteins are mutated or inserted in heterologous contexts, off-pathway reactions can occur resulting in N-terminal, C-terminal, or double cleavage products that are unable to …
Protein splicing: occurrence, mechanisms and related phenomena
1997年3月1日 · Xu eta/. [Zl] proposed two possible mechanisms for protein splicing. One starts with a scrine proteinase-like attack of the downstream serine at the upstream splice junction, followed by the downstream asparagine cyclization and O-N acyl rearrangement. e other mechanism, shown in Figure 2, involves four steps.
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